Polymerase Chain Reaction

 

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Polymerase Chain Reaction

Question 1
Polymerase chain reaction basically consists of
A
two steps
B
three steps
C
four steps
D
five steps

Question 2
The template for PCR is
A
RNA
B
single stranded DNA
C
double stranded DNA
D
none of these

Question 3
Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction?
A
HIV-1 and HIV-2 viruses
B
Hepatitis B virus
C
Mycobacterium tuberculosis
D
All of these

Question 4
The cloning step in PCR/sequencing analysis of microbial communities is necessary for
A
the amplification process
B
preventing contamination by outside DNA
C
separating the different rRNA gene sequences in the mixture
D
none of these

Question 5
RNA populations can also be used as PCR templates after reverse transcription into
A
c DNA
B
t RNA
C
m DNA
D
r RNA

Question 6
The PCR/sequencing approach to rRNA is most likely to give information about
A
the species to which the bacteria are most closely related
B
morphology of the bacterium
C
the type of energy metabolism the bacterium has
D
whether the bacterium is motile

Question 7
The original enzyme(s) used in PCR reaction is/are known as
A
E. coli DNA polymerase
B
E. coli RNA polymerase
C
Taq polymerase
D
all of these

Question 8
The efficiency of amplification in PCR in later cycles is reduced due to
A
reduction in substrate concentration
B
insufficient enzyme and time to synthesize mass quantity of DNA
C
build up of PCR product which competes with primers for hybrid formation
D
all of these
There are 8 questions to complete.

 

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