Pure Cultures

 

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Pure Cultures

Question 1
Which of the following is a function of cryoprotective agents?
A
for long-term preservation of cultures
B
prevents cell damage due to ice crystal formation
C
prevents formation of ice
D
to trap the liquid nitrogen

Question 2
Roll-tube technique is used to isolate .....
A
aerobes
B
anaerobes
C
facultatives
D
stringent anaerobes

Question 3
Which device is used to pick a single bacterial cell from a mixed culture?
A
microscope
B
micropipette
C
microprobe
D
micromanipulator

Question 4
Nichrome loop wire is used in which of the following techniques?
A
Pour-plate
B
Streak-plate
C
Spread-plate
D
Roll-tube technique

Question 5
Which of the following are not performed in lyophilization?
A
agar slant is covered with mineral oil
B
cell suspension is frozen at -60 degree to -78 degree C
C
vials are connected to high-vacuum line
D
bacterial sample is dehydrated

Question 6
What is the temperature of liquid nitrogen?
A
-120 degree C
B
0 degree C
C
-150 degree C
D
-196 degree C

Question 7
Which of the following method can be used to determine the number of bacteria quantitatively?
A
Streak-plate
B
Spread-plate
C
Pour plate
D
Pour-plate and spread plate

Question 8
Dilution of the mixed culture is a common step in all the three methods: the streak-plate, the spread-plate and the pour-plate technique.
A
True
B
False

Question 9
In pour-plate method, the medium should be maintained at what temperature?
A
37 degree C
B
67 degree C
C
45 degree C
D
0 degree C
There are 9 questions to complete.

 

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